By Ado Jorio
This publication covers the advance of biotechnology in accordance with carbon nanostructures, with a spotlight on nanotubes, addressing additionally fullerenes and amorphous carbons. The booklet is split into 7 chapters, addressing tissue engineering, genetic engineering and remedy, in addition to the environmental and well-being affects of carbon nanostructures.
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The prospect that the xenotransplants could provide a solution for the global deﬁcit of transplanting organs and tissues boosted the biomedical researches in this area. The Australian National Heart and Medical Research Council deﬁne xenotransplantation as “the term used to cover the transplantation of living cells, tissues or organs from one species to another” [28, 29]. The biggest challenge to xenotransplant success is the immunological barriers between species. Using transgenic animals with gene insertion or gene knockout is the most promising approach to “fool” rejection mechanisms and incompatibilities between the transplanted organ donor and the recipient .
The oocyte maturation and the meiosis are only concluded with the fertilization process, when the oocyte becomes a zygote. In order for the sperm fertilization to occur, it must ﬁrstly be able to transpose the ZP (aided by enzymes present in its acrosome) and merge with the plasma membrane of the oocyte. After such a fusion, the male genetic material is introduced into the cytoplasm of the oocyte, which expels the second polar corpuscle. The maternal chromosomes (haploid) are then coated with a pro nucleus, while the head of the sperm forms the male pronucleus.
These data correlated with ﬁndings from a previous study  showing that the uptake of SWCNTs did not adversely affect HL60 cells at similar CNT concentration. Thus based on the literature, SWCNTs are considered to be effective carriers of siRNA in a variety of cells, regardless of the amount and type of functionalization used in the SWCNT surface. Fig. 3 CNTs do not affect SKHep 1 cell viability. 025 mg/mL) for 6, 12, 24 and 48 h. a Representative FACS analyses shown in the histogram format.
Bioengineering Applications of Carbon Nanostructures by Ado Jorio