New PDF release: Biomembranes Part N: Transport in Bacteria, Mitochondria,

By Nathan P. Colowick, Nathan P. Kaplan, Sidney Fleischer, Becca Fleischer

ISBN-10: 0121820262

ISBN-13: 9780121820268

The severely acclaimed laboratory normal, Methods in Enzymology, is likely one of the so much hugely revered courses within the box of biochemistry. considering 1955, every one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - really a vital e-book for researchers in all fields of existence sciences

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Extra info for Biomembranes Part N: Transport in Bacteria, Mitochondria, and Chloroplasts: Protonmotive Force

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236, 1850 (1961). 24 D. C. Wharton and A. Tzagoloff, this series, Vol. 10 [45]. 25 L. R. Fowler, S. H. Richardson, and Y. Hatefi, Biochim. Biophys. Acta 96, 103 (1962). 26 C. R. Hartzell, H. Beinert, B. F. van Gelder, and T. E. King, this series, Vol. 53 [10]. z7 M. Saraste, T. Penttil~i, and M. Wikstrrm, Eur. J. Biochem. 115, 261 (1981). 28 T. Pentill~i, Eur. J. Biochem. 133, 355 (1983). 29 R. C. Carroll and E. Racker, J. Biol. Chem. 252, 6981 (1977). 30 B. Ludwig, N. W. Downer, and R. A. Capaldi, Biochemistry 18, 1401 (1979).

The enzyme is dimeric and inserted into a lipid bilayer in the Triton crystals 5 and monomeric and in detergent-rich sheets in the deoxycholate crystals. 7 The enzyme molecule is revealed in both crystal forms as characteristically Y-shaped and divided into three major domains as shown in Fig. 2. The C domain is around 55/~ tall (from the surface of the bilayer) and appears to be contoured into three subdomains. 5,7,~2,~3The Mr domain is larger than the M2 domain, having a surface area in projection of about 900 A compared to 650/~ for M2.

The low-resolution structure is between monomers in the cytochrome-c oxidase dimer 12,13 (Fig. 4). Conclusions and Prospects This brief review shows a picture of mammalian cytochrome-c oxidase built up by combining the data of biochemical studies with a low-resolution structure provided by electron microscopy and image reconstruction. It is now possible to combine the two approaches directly. Recently, electron microscopy has been done using ice rather than phosphotungstate or uranylacetate as the contrast stain for water against protein.

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Biomembranes Part N: Transport in Bacteria, Mitochondria, and Chloroplasts: Protonmotive Force by Nathan P. Colowick, Nathan P. Kaplan, Sidney Fleischer, Becca Fleischer


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