By Professor Patrick F. Dillon
The strength round us -- Molecular contacts -- Diffusion and directed delivery -- strength construction -- strength and flow -- Load bearing -- Fluid and ventilation -- Biophysical interfaces : floor rigidity and membrane structural houses -- Membrane electric houses -- Agonist activation and research -- balance, complexity and non-linear structures
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Additional info for Biophysics: A physiological approach
It is, however, possible to use the electric ﬁeld method described in the previous section, capillary electrophoresis, to measure KD. The use of CE to measure KD employs the following principle: if two molecules form a complex when no electric ﬁeld is applied, and separate in a high electric ﬁeld, then there must be an electric ﬁeld where they are 50% bound and 50% separated. This would allow the measurement of a dissociation constant at that electric ﬁeld, the Ke. By measuring the Ke at several electric ﬁelds and extrapolating to zero electric ﬁeld, the dissociation constant at zero electric ﬁeld, which is the KD, can be determined.
By varying the saturation duration, the values of k1 and k2 can be measured. 11, the control spectrum in the lower ﬁgure showed the unsaturated PCr and γ-ATP peaks in a pig carotid artery, with a control saturation at the asterisks. The upper two spectra show the speciﬁc saturations of the PCr and γ-ATP peaks at the asterisks, totally negating their signals. Note that when ATP is saturated in the upper spectrum, the PCr peak is decreased compared with the control, and that when the PCr peak is saturated in the middle spectrum, the ATP peak is decreased.
For a given charge on a molecule, the smaller the molecule the greater the charge density, and the stronger the electric ﬁeld effect. , 2006). 3 shows CE runs of insulin and glucagon, proteins already known to bind to each other (Root-Bernstein and Dobblestein, 2001). When insulin is present, glucagon shows a concentration-dependent decrease in size; when glucagon is present, insulin shows a concentration-dependent increase in size. This can only occur if the molecules are binding to one another in solution.
Biophysics: A physiological approach by Professor Patrick F. Dillon